Zbtb33 Knockout cell line(4T1)

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location： Home > Products > KO Cell Lines > Zbtb33 Knockout cell line(4T1)

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Zbtb33 Knockout cell line(4T1) Order

Zbtb33 Knockout cell line(4T1)
Catalog#
YKO-M133
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Zbtb33 Knockout cell line(4T1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Zbtb33 Knockout cell line (4T1)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Zbtb33
Gene ID
56805
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%RPMI-1640+10% FBS+1%Sodium Pyruvate+1%Glutamax
Target region
Knockout region: Exon 2 (based on transcript Zbtb33-201)
Validation: PCR+Sanger Sequencing
Zbtb33 Knockout cell line(4T1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:4T1		 STR Info (Cell bank)
Cell Line:4T1
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 241.11
【21.3】		21.3
5-5 335.53
【14】		14.0
6-4 299.38
【18】		18.0
6-7 334.14
【12】		12.0
9-2 220.63
【15】		15.0
12-1 225.61
【16】		16.0
15-3 201.37
【22.3】		22.3
18-3 159.26
【18】		163.73
【19】		18.019.0
X-1 399.75
【25】		25.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Zbtb33 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 4T1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Zbtb33 gene knockout Zbtb33 Knockout cell line (4T1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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